EMPIAR >EMPIAR-10264
LAT1-CD98hc bound to MEM-108 Fab
Author:
Lee Y,Wiriyasermkul P,Jin C,Quan L,Ohgaki R,Okuda S,Kusakizako T,Nishizawa T,Oda K,Ishitani R,Yokoyama T,Nakane T,Shirouzu M,Endou H,Nagamori S,Kanai Y,Nureki O.
Doi:
EMDB entry:
Unaligned multi-frame micrographs, Dataset1
name:
Unaligned multi-frame micrographs, Dataset1
directory:
/data/Dataset1
category:
micrographs - multiframe
headerFormat:
TIFF
dataFormat:
TIFF
numImagesOrTiltSeries:
1582
framesPerImage:
60
voxelType:
SIGNED 16 BIT INTEGER
details:
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. The calibrated pixel size is 0.8346. The total exposure is 46.0 electrons per squared Angstom in a 46.5 second exposure. The data contain a few dozen micrographs that were excluded during the processing due to poor maximal CTF resolution.
dimension-1
imageWidth:
4096
pixelWidth:
0.8346
imageHeight:
4096
pixelHeight:
0.8346
Unaligned multi-frame micrographs, Dataset2
name:
Unaligned multi-frame micrographs, Dataset2
directory:
/data/Dataset2
category:
micrographs - multiframe
headerFormat:
TIFF
dataFormat:
TIFF
numImagesOrTiltSeries:
1900
framesPerImage:
60
voxelType:
SIGNED 16 BIT INTEGER
details:
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. The calibrated pixel size is 0.8346. The total exposure is 46.0 electrons per squared Angstom in a 46.5 second exposure. The data contain a few dozen micrographs that were excluded during the processing due to poor maximal CTF resolution.
dimension-1
imageWidth:
4096
pixelWidth:
0.8346
imageHeight:
4096
pixelHeight:
0.8346
Micelle subtracted particles used for final reconstruction
name:
Micelle subtracted particles used for final reconstruction
directory:
/data/Particles
category:
picked particles - single frame - processed
headerFormat:
MRCS
dataFormat:
MRCS
numImagesOrTiltSeries:
361967
framesPerImage:
1
voxelType:
32 BIT FLOAT
details:
Micelle subtracted particles used for final reconstruction. The MRCS file contains 361,967 particles but some were rejected during classification. The final set of particles and the orientations are stored in the associated STAR file. The image header contains a wrong pixel size information (1.375 A) because of the assumed nominal pixel size (0.861 A). The actual calibrated pixel size is 1.333 A (0.8346 A x 460 pix / 288 pix due to down-sampling), which is used for the final reconstruction in the associated publication.
dimension-1
imageWidth:
288
pixelWidth:
1.333
imageHeight:
288
pixelHeight:
1.333