EMDB >EMD-9849
Single Particle reconstruction
3.31Å
Source organism:
Homo sapiens
[9606]
Mus musculus [10090]
Mus musculus [10090]
Fitted atomic model:
3Dbionotes:
Primary publication:
Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc.
Lee Y, Wiriyasermkul P, Jin C, Quan L, Ohgaki R, Okuda S, Kusakizako T, Nishizawa T, Oda K, Ishitani R, Yokoyama T, Nakane T, Shirouzu M, Endou H, Nagamori S, Kanai Y, Nureki O.
Nat.Struct.Mol.Biol. (2019) 26 pp. 510-517
[doi: 10.1038/s41594-019-0237-7 ]
Lee Y, Wiriyasermkul P, Jin C, Quan L, Ohgaki R, Okuda S, Kusakizako T, Nishizawa T, Oda K, Ishitani R, Yokoyama T, Nakane T, Shirouzu M, Endou H, Nagamori S, Kanai Y, Nureki O.
Nat.Struct.Mol.Biol. (2019) 26 pp. 510-517
[doi: 10.1038/s41594-019-0237-7 ]
PMID:
LAT1-CD98hc complex bound to MEM-108 Fab
Name:
LAT1-CD98hc complex bound to MEM-108 Fab
Type:
PROTEIN
Large neutral amino acids transporter small subunit 1
Name:
Large neutral amino acids transporter small subunit 1
Type:
PROTEIN
Expression system (9606):
Homo sapiens
Theoretical weight (MDa):
0.056
4F2 cell-surface antigen heavy chain
Name:
4F2 cell-surface antigen heavy chain
Type:
PROTEIN
Expression system (9606):
Homo sapiens
Theoretical weight (MDa):
0.068
Antibody
Name:
Antibody
Type:
PROTEIN
Expression system (10090):
Mus musculus
Theoretical weight (MDa):
0.023
Antibody
Name:
Antibody
Type:
PROTEIN
Expression system (10090):
Mus musculus
Theoretical weight (MDa):
0.023
CHOLESTEROL
Name:
CHOLESTEROL
Type:
LIGAND
Theoretical weight (MDa):
0.0
N-ACETYL-D-GLUCOSAMINE
Name:
N-ACETYL-D-GLUCOSAMINE
Type:
LIGAND
Theoretical weight (MDa):
0.0
Sample information
Sample name:
LAT1-CD98hc complex bound to MEM-108 Fab
Ligands:
CHOLESTEROL, N-ACETYL-D-GLUCOSAMINE
Proteins:
LAT1-CD98hc complex bound to MEM-108 Fab, Large neutral amino acids transporter small subunit 1, 4F2 cell-surface antigen heavy chain, Antibody, Antibody
Validation information
Experimental information
Image processing
Number of particles:
250712
Imaging
Session
Microscope model:
FEI_TITAN_KRIOS
Electron source:
FIELD_EMISSION_GUN
Electron dose (e/A**2):
7.14
Holder model:
OTHER
Specimen
Preparation
Specimen state:
PARTICLE
Vitrification
Cryogen:
ETHANE