EMDB >EMD-9850
Single Particle reconstruction
4.1Å
Source organism:
Homo sapiens
[9606]
Mus musculus [10090]
Mus musculus [10090]
Fitted atomic model:
3Dbionotes:
Primary publication:
Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc.
Lee Y, Wiriyasermkul P, Jin C, Quan L, Ohgaki R, Okuda S, Kusakizako T, Nishizawa T, Oda K, Ishitani R, Yokoyama T, Nakane T, Shirouzu M, Endou H, Nagamori S, Kanai Y, Nureki O.
Nat.Struct.Mol.Biol. (2019) 26 pp. 510-517
[doi: 10.1038/s41594-019-0237-7 ]
Lee Y, Wiriyasermkul P, Jin C, Quan L, Ohgaki R, Okuda S, Kusakizako T, Nishizawa T, Oda K, Ishitani R, Yokoyama T, Nakane T, Shirouzu M, Endou H, Nagamori S, Kanai Y, Nureki O.
Nat.Struct.Mol.Biol. (2019) 26 pp. 510-517
[doi: 10.1038/s41594-019-0237-7 ]
PMID:
CD98hc extracellular domain bound to MEM-108 Fab and HBJ127 Fab
Name:
CD98hc extracellular domain bound to MEM-108 Fab and HBJ127 Fab
Type:
PROTEIN
Antibody
Name:
Antibody
Type:
PROTEIN
Expression system (10090):
Mus musculus
Theoretical weight (MDa):
0.024
Antibody
Name:
Antibody
Type:
PROTEIN
Expression system (10090):
Mus musculus
Theoretical weight (MDa):
0.024
4F2 cell-surface antigen heavy chain
Name:
4F2 cell-surface antigen heavy chain
Type:
PROTEIN
Expression system (9606):
Homo sapiens
Theoretical weight (MDa):
0.068
Antibody
Name:
Antibody
Type:
PROTEIN
Expression system (10090):
Mus musculus
Theoretical weight (MDa):
0.023
Antibody
Name:
Antibody
Type:
PROTEIN
Expression system (10090):
Mus musculus
Theoretical weight (MDa):
0.023
N-ACETYL-D-GLUCOSAMINE
Name:
N-ACETYL-D-GLUCOSAMINE
Type:
LIGAND
Theoretical weight (MDa):
0.0
Sample information
Sample name:
CD98hc extracellular domain bound to MEM-108 Fab and HBJ127 Fab
Ligands:
N-ACETYL-D-GLUCOSAMINE
Proteins:
CD98hc extracellular domain bound to MEM-108 Fab and HBJ127 Fab, Antibody, Antibody, 4F2 cell-surface antigen heavy chain, Antibody, Antibody
Validation information
Experimental information
Image processing
Number of particles:
160553
Imaging
Session
Microscope model:
FEI_TECNAI_ARCTICA
Electron source:
FIELD_EMISSION_GUN
Electron dose (e/A**2):
50.2
Holder model:
OTHER
Specimen
Preparation
Specimen state:
PARTICLE
Vitrification
Cryogen:
ETHANE