EMDB >EMD-4738
Single Particle reconstruction 3.5Å
Endogeneous native human 20S proteasome with bound Fabs isolated from less than 1 ul cell lysate
Source organism:
Homo sapiens [9606]
Fitted atomic model:
6r70
3Dbionotes:
available for this entry
Primary publication:
Microfluidic protein isolation and sample preparation for high-resolution cryo-EM.
 Schmidli C, Albiez S, Rima L, Righetto R, Mohammed I, Oliva P, Kovacik L, Stahlberg H, Braun T.
 Proc.Natl.Acad.Sci.USA (2019) 116 pp. 15007-15012
 [doi: 10.1073/pnas.1907214116 ]
PMID:
31292253
Map released:
2019-07-03
Last Modify:
2019-12-18
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Native human 20S proteasome with bound Fabs to the two alpha-4 subunits

Name:
Native human 20S proteasome with bound Fabs to the two alpha-4 subunits
Type:
PROTEIN

Proteasome subunit beta type-4

Name:
Proteasome subunit beta type-4
Type:
PROTEIN
Theoretical weight (MDa):
0.024

Proteasome subunit alpha type-2

Name:
Proteasome subunit alpha type-2
Type:
PROTEIN
Theoretical weight (MDa):
0.026

Proteasome subunit alpha type-4

Name:
Proteasome subunit alpha type-4
Type:
PROTEIN
Theoretical weight (MDa):
0.028

Proteasome subunit alpha type-7

Name:
Proteasome subunit alpha type-7
Type:
PROTEIN
Theoretical weight (MDa):
0.027

Proteasome subunit alpha type-5

Name:
Proteasome subunit alpha type-5
Type:
PROTEIN
Theoretical weight (MDa):
0.025

Proteasome subunit alpha type-1

Name:
Proteasome subunit alpha type-1
Type:
PROTEIN
Theoretical weight (MDa):
0.027

Proteasome subunit alpha type-3

Name:
Proteasome subunit alpha type-3
Type:
PROTEIN
Theoretical weight (MDa):
0.027

Proteasome subunit alpha type-6

Name:
Proteasome subunit alpha type-6
Type:
PROTEIN
Theoretical weight (MDa):
0.027

Proteasome subunit beta type-7

Name:
Proteasome subunit beta type-7
Type:
PROTEIN
Theoretical weight (MDa):
0.024

Proteasome subunit beta type-3

Name:
Proteasome subunit beta type-3
Type:
PROTEIN
Theoretical weight (MDa):
0.023

Proteasome subunit beta type-2

Name:
Proteasome subunit beta type-2
Type:
PROTEIN
Theoretical weight (MDa):
0.022

Proteasome subunit beta type-5

Name:
Proteasome subunit beta type-5
Type:
PROTEIN
Theoretical weight (MDa):
0.022

Proteasome subunit beta type-1

Name:
Proteasome subunit beta type-1
Type:
PROTEIN
Theoretical weight (MDa):
0.024

Proteasome subunit beta type-6

Name:
Proteasome subunit beta type-6
Type:
PROTEIN
Theoretical weight (MDa):
0.022

Sample information

Sample name:
Native human 20S proteasome with bound Fabs to the two alpha-4 subunits
Proteins:
Native human 20S proteasome with bound Fabs to the two alpha-4 subunits, Proteasome subunit beta type-4, Proteasome subunit alpha type-2, Proteasome subunit alpha type-4, Proteasome subunit alpha type-7, Proteasome subunit alpha type-5, Proteasome subunit alpha type-1, Proteasome subunit alpha type-3, Proteasome subunit alpha type-6, Proteasome subunit beta type-7, Proteasome subunit beta type-3, Proteasome subunit beta type-2, Proteasome subunit beta type-5, Proteasome subunit beta type-1, Proteasome subunit beta type-6

Validation information

Visual analysis

Experimental information

Image processing

Number of particles:
16015

Imaging


Session

Microscope model:
FEI_TITAN_KRIOS
Electron source:
FIELD_EMISSION_GUN
Electron dose (e/A**2):
72.0
Holder model:
FEI_TITAN_KRIOS_AUTOGRID_HOLDER

Specimen


Preparation

Specimen state:
PARTICLE
Buffer:
PBS was purchased from Sigma # D8537, Switzerland.

Vitrification

Cryogen:
ETHANE
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