EMDB >EMD-11766
Single Particle reconstruction 3.53Å
Bacterial 30S ribosomal subunit assembly complex state C (body domain)
Source organism:
Escherichia coli [562]
Fitted atomic model:
7afi
3Dbionotes:
available for this entry
Primary publication:
A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.
 Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR.
 Sci Adv (2021) 7
 [doi: 10.1126/sciadv.abf7547 ]
PMID:
34088665
Map released:
2021-07-07
Last Modify:
2021-07-07
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Bacterial 30S ribosomal subunit assembly complex state C (body domain)

Name:
Bacterial 30S ribosomal subunit assembly complex state C (body domain)
Type:
PROTEIN

Ribosomal subunit and RNA

Name:
Ribosomal subunit and RNA
Type:
PROTEIN

Ribosome maturation factor RimP

Name:
Ribosome maturation factor RimP
Type:
PROTEIN
Expression system (562):
Escherichia coli

16SrRNA

Name:
16SrRNA
Type:
NUCLEIC_ACID
Theoretical weight (MDa):
0.5

30S ribosomal protein S4

Name:
30S ribosomal protein S4
Type:
PROTEIN
Theoretical weight (MDa):
0.024

30S ribosomal protein S5

Name:
30S ribosomal protein S5
Type:
PROTEIN
Theoretical weight (MDa):
0.018

30S ribosomal protein S6

Name:
30S ribosomal protein S6
Type:
PROTEIN
Theoretical weight (MDa):
0.016

30S ribosomal protein S8

Name:
30S ribosomal protein S8
Type:
PROTEIN
Theoretical weight (MDa):
0.014

30S ribosomal protein S11

Name:
30S ribosomal protein S11
Type:
PROTEIN
Theoretical weight (MDa):
0.014

30S ribosomal protein S12

Name:
30S ribosomal protein S12
Type:
PROTEIN
Theoretical weight (MDa):
0.014

30S ribosomal protein S15

Name:
30S ribosomal protein S15
Type:
PROTEIN
Theoretical weight (MDa):
0.01

30S ribosomal protein S16

Name:
30S ribosomal protein S16
Type:
PROTEIN
Theoretical weight (MDa):
0.009

30S ribosomal protein S17

Name:
30S ribosomal protein S17
Type:
PROTEIN
Theoretical weight (MDa):
0.01

30S ribosomal protein S18

Name:
30S ribosomal protein S18
Type:
PROTEIN
Theoretical weight (MDa):
0.009

30S ribosomal protein S20

Name:
30S ribosomal protein S20
Type:
PROTEIN
Theoretical weight (MDa):
0.01

Ribosome maturation factor RimP

Name:
Ribosome maturation factor RimP
Type:
PROTEIN
Expression system (562):
Escherichia coli
Theoretical weight (MDa):
0.017

MAGNESIUM ION

Name:
MAGNESIUM ION
Type:
LIGAND
Theoretical weight (MDa):
0.0

Sample information

Sample name:
Bacterial 30S ribosomal subunit assembly complex state C (body domain)
Ligands:
MAGNESIUM ION
Proteins:
Bacterial 30S ribosomal subunit assembly complex state C (body domain), Ribosomal subunit and RNA, Ribosome maturation factor RimP, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S8, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S20, Ribosome maturation factor RimP

Validation information

Visual analysis

Experimental information

Image processing

Number of particles:
14097

Imaging


Session

Microscope model:
FEI_TITAN_KRIOS
Electron source:
FIELD_EMISSION_GUN
Electron dose (e/A**2):
46.1
Holder model:
FEI_TITAN_KRIOS_AUTOGRID_HOLDER

Specimen


Preparation

Specimen state:
PARTICLE

Vitrification

Apparatus:
FEI_VITROBOT_MARK_III
Cryogen:
ETHANE
Humidity:
100
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