EMDB >EMD-11761
Single Particle reconstruction
3.44Å
Source organism:
Escherichia coli
[562]
Fitted atomic model:
3Dbionotes:
Primary publication:
A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR.
Sci Adv (2021) 7
[doi: 10.1126/sciadv.abf7547 ]
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR.
Sci Adv (2021) 7
[doi: 10.1126/sciadv.abf7547 ]
PMID:
Bacterial 30S ribosomal subunit assembly complex state A (head domain)
Name:
Bacterial 30S ribosomal subunit assembly complex state A (head domain)
Type:
PROTEIN
16SrRNA of the head domain (residue C931 to G1386)
Name:
16SrRNA of the head domain (residue C931 to G1386)
Type:
NUCLEIC_ACID
Theoretical weight (MDa):
0.5
30S ribosomal protein S2
Name:
30S ribosomal protein S2
Type:
PROTEIN
Theoretical weight (MDa):
0.027
30S ribosomal protein S3
Name:
30S ribosomal protein S3
Type:
PROTEIN
Theoretical weight (MDa):
0.026
30S ribosomal protein S7
Name:
30S ribosomal protein S7
Type:
PROTEIN
Theoretical weight (MDa):
0.02
30S ribosomal protein S9
Name:
30S ribosomal protein S9
Type:
PROTEIN
Theoretical weight (MDa):
0.015
30S ribosomal protein S10
Name:
30S ribosomal protein S10
Type:
PROTEIN
Theoretical weight (MDa):
0.012
30S ribosomal protein S13
Name:
30S ribosomal protein S13
Type:
PROTEIN
Theoretical weight (MDa):
0.013
30S ribosomal protein S14
Name:
30S ribosomal protein S14
Type:
PROTEIN
Theoretical weight (MDa):
0.012
30S ribosomal protein S19
Name:
30S ribosomal protein S19
Type:
PROTEIN
Theoretical weight (MDa):
0.01
MAGNESIUM ION
Name:
MAGNESIUM ION
Type:
LIGAND
Theoretical weight (MDa):
0.0
ZINC ION
Name:
ZINC ION
Type:
LIGAND
Theoretical weight (MDa):
0.0
Sample information
Sample name:
Bacterial 30S ribosomal subunit assembly complex state A (head domain)
Ligands:
MAGNESIUM ION, ZINC ION
Proteins:
Bacterial 30S ribosomal subunit assembly complex state A (head domain), 30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S7, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S13, 30S ribosomal protein S14, 30S ribosomal protein S19
Validation information
Experimental information
Image processing
Number of particles:
19301
Imaging
Session
Microscope model:
FEI_TITAN_KRIOS
Electron source:
FIELD_EMISSION_GUN
Electron dose (e/A**2):
46.1
Holder model:
FEI_TITAN_KRIOS_AUTOGRID_HOLDER
Specimen
Preparation
Specimen state:
PARTICLE
Vitrification
Apparatus:
FEI_VITROBOT_MARK_III
Cryogen:
ETHANE
Humidity:
100