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EMDB >EMD-
11312
Helical reconstruction
3.6Å
Structure of the Shigella MxiH needle filament attached to the basal body
Source organism:
Shigella flexneri 5a str. M90T
[
1086030
]
Fitted atomic model:
6zni
3Dbionotes:
available for this entry
Primary publication:
Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies
Kotov V, Lunelli M, Wald J, Kolbe M, Marlovits TC.
Biochem Biophys Rep (2021)
[doi:
10.1016/j.bbrep.2021.101039
]
Map released:
2021-07-07
Last Modify:
2021-07-07
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Function and Biology
Experimental Information
Needle filament of the type III secretion system
Name:
Needle filament of the type III secretion system
Type:
CELLULAR_COMPONENT
Protein MxiH
Name:
Protein MxiH
Type:
PROTEIN
Expression system (
1086030
):
Shigella flexneri 5a str. M90T
Theoretical weight (
MDa
):
0.011
Sample information
Sample name:
Needle filament of the type III secretion system
Proteins:
Protein MxiH
Validation information
Visual analysis
Experimental information
Image processing
Imaging
Session
Microscope model:
FEI_TITAN_KRIOS
Electron source:
FIELD_EMISSION_GUN
Electron dose (
e/A**2
):
25.0
Holder model:
FEI_TITAN_KRIOS_AUTOGRID_HOLDER
Specimen
Preparation
Specimen state:
PARTICLE
Vitrification
Apparatus:
FEI_VITROBOT_MARK_IV
Cryogen:
OTHER
Humidity:
100