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EMDB >EMD-
0690
Single Particle reconstruction
3.3Å
The reconstruction of apo-state streptavidin at 3.3 Angstrom resolution
Source organism:
Streptomyces avidinii
[
1895
]
Fitted atomic model:
6j6k
3Dbionotes:
available for this entry
Primary publication:
Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.
Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW.
Nat Commun (2019) 10 pp. 2386-2386
[doi:
10.1038/s41467-019-10368-w
]
PMID:
31160591
Map released:
2019-05-29
Last Modify:
2020-08-05
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Function and Biology
Experimental Information
Streptavidin
Name:
Streptavidin
Type:
PROTEIN
Streptavidin
Name:
Streptavidin
Type:
PROTEIN
Theoretical weight (
MDa
):
0.013
Sample information
Sample name:
Streptavidin
Proteins:
Streptavidin, Streptavidin
Validation information
Visual analysis
Experimental information
Image processing
Number of particles:
23991
Imaging
Session
Microscope model:
FEI_TITAN_KRIOS
Electron source:
FIELD_EMISSION_GUN
Electron dose (
e/A**2
):
50.0
Holder model:
FEI_TITAN_KRIOS_AUTOGRID_HOLDER
Specimen
Preparation
Specimen concentration(
mg/ml
):
0.2
Specimen state:
PARTICLE
Vitrification
Apparatus:
FEI_VITROBOT_MARK_IV
Cryogen:
ETHANE
Humidity:
100