EMDB >EMD-0689
Single Particle reconstruction
3.2Å
Source organism:
Streptomyces avidinii
[1895]
Fitted atomic model:
3Dbionotes:
Primary publication:
Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.
Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW.
Nat Commun (2019) 10 pp. 2386-2386
[doi: 10.1038/s41467-019-10368-w ]
Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW.
Nat Commun (2019) 10 pp. 2386-2386
[doi: 10.1038/s41467-019-10368-w ]
PMID:
Streptavidin with biotin
Name:
Streptavidin with biotin
Type:
PROTEIN
Streptavidin
Name:
Streptavidin
Type:
PROTEIN
Theoretical weight (MDa):
0.013
BIOTIN
Name:
BIOTIN
Type:
LIGAND
Theoretical weight (MDa):
0.0
water
Name:
water
Type:
LIGAND
Theoretical weight (MDa):
0.0
Sample information
Sample name:
Streptavidin with biotin
Ligands:
BIOTIN, water
Proteins:
Streptavidin with biotin, Streptavidin
Validation information
Experimental information
Image processing
Number of particles:
45686
Imaging
Session
Microscope model:
FEI_TITAN_KRIOS
Electron source:
FIELD_EMISSION_GUN
Electron dose (e/A**2):
50.0
Holder model:
FEI_TITAN_KRIOS_AUTOGRID_HOLDER
Specimen
Preparation
Specimen concentration(mg/ml):
0.2
Specimen state:
PARTICLE
Vitrification
Apparatus:
FEI_VITROBOT_MARK_IV
Cryogen:
ETHANE
Humidity:
100