EMDB >EMD-5174
Single Particle reconstruction
18.0Å
Source organism:
Escherichia coli
[562]
3Dbionotes:
Primary publication:
Structure of the 100S ribosome in the hibernation stage revealed by electron cryomicroscopy.
Kato T, Yoshida H, Miyata T, Maki Y, Wada A, Namba K.
STRUCTURE (2010) 18 pp. 719-724
[doi: 10.1016/j.str.2010.02.017 ]
Kato T, Yoshida H, Miyata T, Maki Y, Wada A, Namba K.
STRUCTURE (2010) 18 pp. 719-724
[doi: 10.1016/j.str.2010.02.017 ]
PMID:
100S ribosome
Name:
100S ribosome
Type:
RIBOSOME_PROKARYOTE
Sample information
Sample name:
E. coli 100S ribosome
Validation information
Experimental information
Image processing
Number of particles:
17461
Imaging
Session
Microscope model:
JEOL_3200_FSC
Electron source:
FIELD_EMISSION_GUN
Electron dose (e/A**2):
20.0
Energy filter:
JEOL Omega filter
Holder model:
JEOL
Specimen
Preparation
Specimen state:
PARTICLE
Buffer:
20 mM HEPES-KCL, 77 mM KCl, 15 mM (CH3COO)2Mg
Vitrification
Apparatus:
FEI_VITROBOT
Cryogen:
ETHANE
Humidity:
90